CD68 Monoclonal antibody
CD68 Monoclonal Antibody for IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
3A9A7
Cat no : 66231-2-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human tonsillitis tissue, human lung cancer tissue, human liver cancer tissue, human urothelial carcinoma tissue, human appendicitis tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1000-1:6000 |
| Immunofluorescence (IF) | IF : 1:3000-1:12000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 7 publications below |
| IHC | See 15 publications below |
| IF | See 17 publications below |
| FC | See 2 publications below |
Product Information
66231-2-Ig targets CD68 in IHC, IF, FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD68 fusion protein Ag22815 |
| Full Name | CD68 molecule |
| Calculated molecular weight | 37 kDa |
| GenBank accession number | BC015557 |
| Gene symbol | CD68 |
| Gene ID (NCBI) | 968 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD68 antibody 66231-2-Ig | Download protocol |
| IF protocol for CD68 antibody 66231-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest Lactate inhibits ATP6V0d2 expression in tumor-associated macrophages to promote HIF-2α-mediated tumor progression. | ||
Redox Biol Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury. | ||
Transl Res Purinergic receptor P2 × 7 contributes to abdominal aortic aneurysm development via modulating macrophage pyroptosis and inflammation | ||
Cancer Lett SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer. | ||
Carbohydr Polym Multifunctional chitin-based hollow nerve conduit for peripheral nerve regeneration and neuroma inhibition. | ||
Cancers (Basel) The Immunological Contribution of a Novel Metabolism-Related Signature to the Prognosis and Anti-Tumor Immunity in Cervical Cancer. |
