CD68 Monoclonal antibody

CD68 Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3A9A7

Cat no : 66231-2-Ig

Synonyms

CD68, CD68 molecule, GP110, Macrosialin, SCARD1



Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue, human liver cancer tissue, human urothelial carcinoma tissue, human appendicitis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:3000-1:12000
Sample-dependent, check data in validation data gallery

Product Information

66231-2-Ig targets CD68 in IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD68 fusion protein Ag22815
Full Name CD68 molecule
Calculated molecular weight 37 kDa
GenBank accession numberBC015557
Gene symbol CD68
Gene ID (NCBI) 968
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Protocols

Product Specific Protocols
IHC protocol for CD68 antibody 66231-2-IgDownload protocol
IF protocol for CD68 antibody 66231-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Clin Invest

Lactate inhibits ATP6V0d2 expression in tumor-associated macrophages to promote HIF-2α-mediated tumor progression.

Authors - Na Liu
mouseIHC

Redox Biol

Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury.

Authors - Ming Kong
humanIF

Transl Res

Purinergic receptor P2 × 7 contributes to abdominal aortic aneurysm development via modulating macrophage pyroptosis and inflammation

Authors - Likun Sun
humanIHC,IF,FC

Cancer Lett

SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer.

Authors - Tian-Jiao Li
ratIHC

Carbohydr Polym

Multifunctional chitin-based hollow nerve conduit for peripheral nerve regeneration and neuroma inhibition.

Authors - Xiaqing Yang
humanIHC

Cancers (Basel)

The Immunological Contribution of a Novel Metabolism-Related Signature to the Prognosis and Anti-Tumor Immunity in Cervical Cancer.

Authors - Sihui Yu