CD68 Monoclonal antibody

CD68 Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3A9A7

Cat no : 66231-2-Ig

Synonyms

CD68, CD68 molecule, GP110, Macrosialin, SCARD1



Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue, human liver cancer tissue, human urothelial carcinoma tissue, human appendicitis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:6000
Immunofluorescence (IF)-PIF-P : 1:3000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66231-2-Ig targets CD68 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD68 fusion protein Ag22815
Full Name CD68 molecule
Calculated Molecular Weight 37 kDa
GenBank Accession NumberBC015557
Gene Symbol CD68
Gene ID (NCBI) 968
RRIDAB_2881622
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Protocols

Product Specific Protocols
IHC protocol for CD68 antibody 66231-2-IgDownload protocol
IF protocol for CD68 antibody 66231-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

J Clin Invest

Lactate inhibits ATP6V0d2 expression in tumor-associated macrophages to promote HIF-2α-mediated tumor progression.

Authors - Na Liu
IF

Adv Healthc Mater

Wireless Electric Cues Mediate Autologous DPSC-Loaded Conductive Hydrogel Microspheres to Engineer The Immuno-Angiogenic Niche for Homologous Maxillofacial Bone Regeneration

Authors - Jiwei Sun
IHC

Redox Biol

Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury.

Authors - Ming Kong
humanIF

Transl Res

Purinergic receptor P2 × 7 contributes to abdominal aortic aneurysm development via modulating macrophage pyroptosis and inflammation

Authors - Likun Sun
IF

Diabetes

Macrophage SHP2 Deficiency Alleviates Diabetic Nephropathy via Suppression of MAPK/NF-κB- Dependent Inflammation

Authors - Xue Han
humanIHC,IF,FC

Cancer Lett

SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer.

Authors - Tian-Jiao Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Balawant (Verified Customer) (12-25-2023)

This excellent antibody has no background.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: colon tissue