CoraLite® Plus 647 Anti-Human CD62P (AK4) Mouse IgG2a Recombinant Antibody

CD62P Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

AK4

Cat no : CL647-65587

Synonyms

P-selectin, GMRP, GMP-140, GMP140, GMP 140

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Validation Data Gallery

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  FC experiment of human peripheral blood platelets using CL647-65587

  1x10^6 thrombin treated human peripheral blood platelets were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD62P (AK4) Mouse IgG2a Recombinant Antibody (CL647-65587, Clone: AK4) (red), or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4) (blue). 1x10^6 untreated human peripheral blood platelets were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD62P (AK4) Mouse IgG2a Recombinant Antibody (CL647-65587, Clone: AK4) (black). Cells were not fixed.

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Tested Applications

• Positive FC detected in: Thrombin treated human peripheral blood platelets

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65587 targets CD62P in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human platelets
• Full Name: selectin P (granule membrane protein 140kDa, antigen CD62)
• Calculated Molecular Weight: 830 aa, 91 kDa
• GenBank Accession Number: BC028067
• Gene Symbol: CD62P
• Gene ID (NCBI): 6403
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.