Biotin Anti-Human CD62P (AK4) Mouse IgG2a Recombinant Antibody

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  FC experiment of human peripheral blood platelets using Biotin-65587

  1x10^6 thrombin-treated human peripheral blood platelets were surface stained with 5 ul Biotin Plus Anti-Human CD62P (AK4) Mouse IgG2a RecAb (Biotin-65587, Clone: AK4) and CoraLite®488-conjugated streptavidin (red) or unstained cells (blue). 1x10^6 untreated human peripheral blood platelets were surface stained with 5 ul Biotin Plus Anti-Human CD62P (AK4) Mouse IgG2a RecAb (Biotin-65587, Clone: AK4) and CoraLite®488-conjugated streptavidin (black, dashed). Cells were not fixed.

CD62P Uni-rAb^TM Recombinant Antibody for FC

Cat No. Biotin-65587

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

P-selectin, GMRP, GMP-140, GMP140, GMP 140

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Tested Applications

• Positive FC detected in: Thrombin treated human peripheral blood platelets

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65587 targets CD62P in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human platelets
• Full Name: selectin P (granule membrane protein 140kDa, antigen CD62)
• Calculated Molecular Weight: 830 aa, 91 kDa
• GenBank Accession Number: BC028067
• Gene Symbol: CD62P
• Gene ID (NCBI): 6403
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.