CD59 Recombinant antibody, PBS Only

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  WB analysis using 84447-1-RR (same clone as 84447-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84447-1-RR (CD59 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84447-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 84447-1-RR (same clone as 84447-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using CD59 antibody (84447-1-RR, Clone: 241310G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84447-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84447-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84447-1-RR against Human CD59 were performed. The affinity constant is 1.59 nM.

CD59 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84447-1-PBS

Clone No.241310G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 241310G8

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Product Information

84447-1-PBS targets CD59 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: CD59 molecule, complement regulatory protein
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC001506
• Gene Symbol: CD59
• Gene ID (NCBI): 966
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P13987
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.