CD59 Recombinant antibody

CD59 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241310G8

Cat no : 84447-1-RR

Synonyms

HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 241310G8

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Validation Data Gallery

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  WB analysis using 84447-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84447-1-RR (CD59 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of BxPC-3 using 84447-1-RR

  Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using CD59 antibody (84447-1-RR, Clone: 241310G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84447-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84447-1-RR against Human CD59 were performed. The affinity constant is 1.59 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, HUVEC cells, BxPC-3 cells
• Positive IF/ICC detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84447-1-RR targets CD59 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: CD59 molecule, complement regulatory protein
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC001506
• Gene Symbol: CD59
• Gene ID (NCBI): 966
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.