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CD59 Recombinant antibody

CD59 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241310G8

Cat no : 84447-1-RR

Synonyms

HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 241310G8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD59 Recombinant antibody (84447-1-RR)

    WB analysis using 84447-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84447-1-RR (CD59 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using CD59 Recombinant antibody (84447-1-RR)

    IF Staining of BxPC-3 using 84447-1-RR

    Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using CD59 antibody (84447-1-RR, Clone: 241310G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84447-1-RR

    Affinity and Kinetic Characterization of 84447-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84447-1-RR against Human CD59 were performed. The affinity constant is 1.59 nM.

Tested Applications

Positive WB detected inHeLa cells, HUVEC cells, BxPC-3 cells
Positive IF/ICC detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84447-1-RR targets CD59 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name CD59 molecule, complement regulatory protein
Calculated Molecular Weight 19 kDa
Observed Molecular Weight18-20 kDa
GenBank Accession NumberBC001506
Gene Symbol CD59
Gene ID (NCBI) 966
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

Protocols

Product Specific Protocols
WB protocol for CD59 antibody 84447-1-RRDownload protocol
IF protocol for CD59 antibody 84447-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols