Anti-Human CD58 (TS2/9) Mouse IgG2a Recombinant Antibody, PBS Only

CD58 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

TS2/9

Cat no : 65597-1-PBS

Synonyms

Surface glycoprotein LFA 3, Lymphocyte function-associated antigen 3, LFA3, LFA 3, CD58 molecule

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Validation Data Gallery

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  FC experiment of human peripheral blood leukocytes using 65597-1-MR (same clone as 65597-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD58 (TS2/9) Mouse IgG2a Recombinant Antibody (65597-1-MR, Clone: TS2/9) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65597-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 65597-1-MR (same clone as 65597-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD58 (TS2/9) Mouse IgG2a Recombinant Antibody (65597-1-MR, Clone: TS2/9) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65597-1-PBS in a different storage buffer formulation.

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Product Information

65597-1-PBS targets CD58 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human cytolytic T cells
• Full Name: CD58 molecule
• Calculated Molecular Weight: 28 kDa
• GenBank Accession Number: BC005930
• Gene Symbol: CD58
• Gene ID (NCBI): 965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.