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Anti-Human CD58 (TS2/9) Mouse IgG2a Recombinant Antibody

CD58 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

TS2/9

Cat no : 65597-1-MR

Synonyms

Surface glycoprotein LFA 3, Lymphocyte function-associated antigen 3, LFA3, LFA 3, CD58 molecule

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CD58 (TS2/9) Mouse IgG2a Recombinant An (65597-1-MR)

    FC experiment of human peripheral blood leukocytes using 65597-1-MR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD58 (TS2/9) Mouse IgG2a Recombinant Antibody (65597-1-MR, Clone: TS2/9) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CD58 (TS2/9) Mouse IgG2a Recombinant An (65597-1-MR)

    FC experiment of human peripheral blood leukocytes using 65597-1-MR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD58 (TS2/9) Mouse IgG2a Recombinant Antibody (65597-1-MR, Clone: TS2/9) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Tested Applications

Positive FC detected inhuman peripheral blood leukocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65597-1-MR targets CD58 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human cytolytic T cells
Full Name CD58 molecule
Calculated Molecular Weight 28 kDa
GenBank Accession NumberBC005930
Gene Symbol CD58
Gene ID (NCBI) 965
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.

Protocols

Product Specific Protocols
FC protocol for CD58 antibody 65597-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols