CoraLite® Plus 488 Anti-Mouse CD48 (HM48-1)

CD48 Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

HM48-1

Cat no : CL488-65227

Synonyms

MEM 102, HM48-1, CD48 antigen, BLAST1, BLAST 1

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Validation Data Gallery

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  FC experiment of mouse splenocytes using CL488-65227

  1X10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD48 (CL488-65227, Clone:HM48-1) (red), or 0.5 ug Isotype Control. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65227 targets CD48 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse T lymphoma MBL-2
• Full Name: CD48 antigen
• GenBank Accession Number: BC060977
• Gene Symbol: Cd48
• Gene ID (NCBI): 12506
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.