PerCP-Cyanine5.5 Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant Antibody

CD45RO Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

PerCP-Cyanine5.5 Fluorescent Dye

CloneNo.

UCHL1

Cat no : CPY5-65613

Synonyms

CD45, B220, LCA, L CA, GP180

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Validation Data Gallery

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  FC experiment of human PBMCs using CPY5-65613

  1x10^6 human PBMCs were surface stained with FITC Plus Anti-Human CD45RA and 5 ul PerCP-Cyanine5.5 Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant Antibody (CPY5-65613, Clone:UCHL1) or unstained. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CPY5-65613

  1x10^6 human PBMCs were surface stained with 5 ul PerCP-Cyanine5.5 Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant Antibody (CPY5-65613, Clone:UCHL1) (red) or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CPY5-65613 targets CD45RO in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: IL-2 dependent T cell line, CA1
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC014239
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• Conjugate: PerCP-Cyanine5.5 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 482 nm / 690nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).