Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant Antibody

CD45RO Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

UCHL1

Cat no : 65613-1-MR

Synonyms

CD45, B220, L CA, GP180, EC:3.1.3.48

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Validation Data Gallery

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  FC experiment of human PBMCs using 65613-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45RO Mouse IgG2a Recombinant Antibody (65613-1-MR, Clone: UCHL1) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD45RA. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65613-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45RO Mouse IgG2a Recombinant Antibody (65613-1-MR, Clone: UCHL1) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65613-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45RO Mouse IgG2a Recombinant Antibody (65613-1-MR, Clone: UCHL1) (left) or 0.25 ug Anti-Human CD45RO (65150-1-Ig, Clone: UCHL1) (right) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD45RA. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65613-1-MR targets CD45RO in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: IL-2 dependent T cell line, CA1
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC014239
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).