Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant Antibody, PBS Only

CD45RO Uni-rAb^TM Recombinant Antibody for IHC, FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, FC

Conjugate

Unconjugated

CloneNo.

UCHL1

Cat no : 65613-1-PBS

Synonyms

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Validation Data Gallery

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  IHC staining of human tonsillitis using 65613-1-MR (same clone as 65613-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65613-1-MR (CD45RO antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 65613-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 65613-1-MR (same clone as 65613-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65613-1-MR (CD45RO antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 65613-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 65613-1-MR (same clone as 65613-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45RO Mouse IgG2a Recombinant Antibody (65613-1-MR, Clone: UCHL1) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD45RA. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65613-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65613-1-MR (same clone as 65613-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45RO Mouse IgG2a Recombinant Antibody (65613-1-MR, Clone: UCHL1) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65613-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65613-1-MR (same clone as 65613-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45RO Mouse IgG2a Recombinant Antibody (65613-1-MR, Clone: UCHL1) (left) or 0.25 ug Anti-Human CD45RO (65150-1-Ig, Clone: UCHL1) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD45RA. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65613-1-PBS in a different storage buffer formulation.

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Product Information

65613-1-PBS targets CD45RO in IHC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: IL-2 dependent T cell line, CA1
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC014239
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).