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Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant Antibody

CD45RO Uni-rAbTM Recombinant Antibody for IHC, FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, FC

Conjugate

Unconjugated

CloneNo.

UCHL1

Cat no : 65613-1-MR

Synonyms

CD45, B220, LCA, L CA, GP180

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant (65613-1-MR)

    IHC staining of human tonsillitis using 65613-1-MR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65613-1-MR (CD45RO antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant (65613-1-MR)

    IHC staining of human tonsillitis using 65613-1-MR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65613-1-MR (CD45RO antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant (65613-1-MR)

    FC experiment of human PBMCs using 65613-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45RO Mouse IgG2a Recombinant Antibody (65613-1-MR, Clone: UCHL1) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD45RA. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant (65613-1-MR)

    FC experiment of human PBMCs using 65613-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45RO Mouse IgG2a Recombinant Antibody (65613-1-MR, Clone: UCHL1) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant (65613-1-MR)

    FC experiment of human PBMCs using 65613-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD45RO Mouse IgG2a Recombinant Antibody (65613-1-MR, Clone: UCHL1) (left) or 0.25 ug Anti-Human CD45RO (65150-1-Ig, Clone: UCHL1) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD45RA. Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:600-1:2400
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65613-1-MR targets CD45RO in IHC, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen IL-2 dependent T cell line, CA1
Full Name protein tyrosine phosphatase, receptor type, C
GenBank Accession NumberBC014239
Gene Symbol CD45
Gene ID (NCBI) 5788
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).

Protocols

Product Specific Protocols
IHC protocol for CD45RO antibody 65613-1-MRDownload protocol
FC protocol for CD45RO antibody 65613-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols