PE Anti-Rat CD45RA (OX-33) Mouse IgG2a Recombinant Antibody

CD45RA Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

rat

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

OX-33

Cat no : PE-65578

Synonyms

Lca, Ptprc, CD45, RT7, OX-33

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Validation Data Gallery

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  FC experiment of rat splenocytes using PE-65578

  1x10^6 rat splenocytes were surface stained with APC Anti-Rat CD4 and 0.25 ug PE Anti-Rat CD45RA (OX-33) Mouse IgG2a Recombinant Antibody (PE-65578, Clone: OX-33) (right), or PE Mouse IgG2a Isotype Control (C1.18.4) (PE-65208, Clone: C1.18.4) (left). Cells were not fixed.

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Tested Applications

• Positive FC detected in: rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65578 targets CD45RA in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Leukocyte common antigen purified from rat splenocytes
• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 143 kDa
• GenBank Accession Number: XM_006249910
• Gene Symbol: CD45
• Gene ID (NCBI): 24699
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signaling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).