CoraLite® Plus 647 Anti-Rat CD45RA (OX-33) Mouse IgG1 Recombinant Antibody

CD45RA Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Rat

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

OX-33

Cat no : CL647-65543

Synonyms

CD45, L CA, Lca, Ptprc, RT7, T200

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Validation Data Gallery

• 

  FC experiment of rat splenocytes using CL647-65543

  1x10^6 rat splenocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Rat CD45RA (OX-33) Mouse Recombinant Antibody (CL647-65543, Clone:OX-33) or 0.5 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21), and FITC Plus Anti-Rat CD4 (OX-35) (FITC-65179, Clone: OX-35). Cells were not fixed.

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Tested Applications

• Positive FC detected in: rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65543 targets CD45RA in FC applications and shows reactivity with Rat samples.

• Tested Reactivity: Rat
• Host / Isotype: Mouse / IgG1
• Class: Recombinant
• Type: Antibody
• Immunogen: Leukocyte common antigen purified from rat splenocytes
• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 143 kDa
• GenBank Accession Number: XM_006249910
• Gene Symbol: CD45RA
• Gene ID (NCBI): 24699
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).