CoraLite® Plus 405 Anti-Mouse CD45R (B220) (RA3-6B2)

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  FC experiment of mouse splenocytes using CL405-65139

  1x10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD3, and 0.25 ug CoraLite® Plus 405 Anti-Mouse CD45R (B220) (RA3-6B2) (CL405-65139, Clone: RA3-6B2) or CoraLite® Plus 405 Rat IgG2a Isotype Control (2A3) (CL405-65209, Clone: 2A3). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL405-65139

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 405 Anti-Mouse CD45R (B220) (RA3-6B2) (CL405-65139, Clone: RA3-6B2) (red) or CoraLite® Plus 405 Rat IgG2a Isotype Control (2A3) (CL405-65209, Clone: 2A3) (blue). Cells were not fixed.

CD45R (B220) Monoclonal Antibody for FC

Cat No. CL405-65139

Clone No.RA3-6B2

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC

Synonyms

B220, CD45R, Cd45, Ptprc, EC:3.1.3.48

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL405-65139 targets CD45R (B220) in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Abelson murine leukemia virus-induced pre-B tumor cells

• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC028512
• Gene Symbol: CD45
• Gene ID (NCBI): 19264
• RRID: AB_2934361
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P06800
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.