Anti-Mouse CD45R (B220) (RA3-6B2)

CD45R (B220) Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2a, kappa


Human, Mouse and More (1)







Cat no : 65139-1-Ig


B220, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

Tested Applications

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

Immunofluorescence (IF)IF : 1:250-1:1000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

65139-1-Ig targets CD45R (B220) in IF, FC applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivitymouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Abelson murine leukemia virus-induced pre-B tumor cells
Full Name protein tyrosine phosphatase, receptor type, C
GenBank Accession NumberBC028512
Gene Symbol Ptprc
Gene ID (NCBI) 19264
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.


Product Specific Protocols
IF protocol for CD45R (B220) antibody 65139-1-IgDownload protocol
FC protocol for CD45R (B220) antibody 65139-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Thorac Cancer

Experimental model of micronodular thymic neoplasm with lymphoid stroma

Authors - Riko Niwa