Anti-Mouse CD45R (B220) (RA3-6B2)

CD45R (B220) Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF/ICC, FC

Conjugate

Unconjugated

Publications

1

CloneNo.

RA3-6B2

Cat no : 65139-1-Ig

Synonyms

Cd45, Ptprc, RA3-6B2, Leukocyte common antigen, LCA

---

Validation Data Gallery

• 

  IF Staining of mouse splenocytes using 65139-1-Ig

  Immunofluorescent analysis of mouse splenocytes using CD45R (B220) antibody (65139-1-Ig, Clone: RA3-6B2 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rat IgG(H+L).

• 

  FC experiment of C57BL/c mouse splenocytes using 65139-1-Ig

  1X10^6 C57BL/c mouse splenocytes were surface co-stained with APC Anti-Mouse CD3 and 0.5 ug Anti-Mouse CD45R (B220) (65139-1-Ig, Clone: RA3-6B2) or 0.5 ug Isotype Control. Cells were not fixed.

---

Tested Applications

• Positive IF/ICC detected in: mouse splenocytes
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

65139-1-Ig targets CD45R (B220) in IF/ICC, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Abelson murine leukemia virus-induced pre-B tumor cells
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC028512
• Gene Symbol: CD45
• Gene ID (NCBI): 19264
• RRID: AB_2918426
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.

Publications

Species	Application	Title
mouse	IF	Thorac Cancer; Experimental model of micronodular thymic neoplasm with lymphoid stroma; Authors - Riko Niwa