PE Anti-Mouse CD45 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using PE-98035

  1x10^6 mouse splenocytes were surface stained with 0.25 ug PE Anti-Mouse CD45 Rabbit Recombinant Antibody (PE-98035, Clone: 240356D1) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PE-98035

  1x10^6 mouse splenocytes were surface stained with 0.25 ug PE Anti-Mouse CD45 Rabbit Recombinant Antibody (PE-98035, Clone:240356D1) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

CD45 Uni-rAb^TM Recombinant Antibody for FC

Cat No. PE-98035

Clone No.240356D1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Ptprc, Leukocyte common antigen, LCA, B220, 240356D1

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-98035 targets CD45 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 145 kDa
• GenBank Accession Number: NM_001111316.2
• Gene Symbol: CD45
• Gene ID (NCBI): 19264
• RRID: AB_3674017
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06800-4
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).