FC experiment of mouse splenocytes using 98035-1-RR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45 Rabbit Recombinant Antibody (98035-1-RR, Clone: 240356D1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45 Rabbit Recombinant Antibody (98035-1-RR, Clone: 240356D1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
FC experiment of mouse splenocytes using 98035-1-RR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45 Rabbit Recombinant Antibody (98035-1-RR, Clone: 240356D1) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45 Rabbit Recombinant Antibody (98035-1-RR, Clone: 240356D1) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
Affinity and Kinetic Characterization of 98035-1-RR
Biolayer interferometry (BLl) kinetic assays of 98035-1 against Mouse CD45 were performed. The affinity constant is 0.787 nM.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98035-1-RR targets CD45 in FC applications and shows reactivity with mouse samples.
Store at 2-8°C. Stable for one year after shipment.
Background Information
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
FC experiment of mouse splenocytes using 98035-1-RR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45 Rabbit Recombinant Antibody (98035-1-RR, Clone: 240356D1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
FC experiment of mouse splenocytes using 98035-1-RR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45 Rabbit Recombinant Antibody (98035-1-RR, Clone: 240356D1) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
AFFINITY Figures
Affinity and Kinetic Characterization of 98035-1-RR
Biolayer interferometry (BLl) kinetic assays of 98035-1 against Mouse CD45 were performed. The affinity constant is 0.787 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD45 Recombinant antibody
Catalog Number
98035-1-RR
Citations
-
Dilutions
Applications
FC
Reactivity
mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
