Anti-Mouse CD45 Rabbit Recombinant Antibody

CD45 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240356D1

Cat no : 98035-1-RR

Synonyms

Ptprc, Leukocyte common antigen, LCA, B220, 240356D1

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using 98035-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45 Rabbit Recombinant Antibody (98035-1-RR, Clone: 240356D1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98035-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45 Rabbit Recombinant Antibody (98035-1-RR, Clone: 240356D1) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98035-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98035-1 against Mouse CD45 were performed. The affinity constant is 0.787 nM.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98035-1-RR is CD45 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 145 kDa
• GenBank Accession Number: NM_001111316.2
• Gene Symbol: CD45
• Gene ID (NCBI): 19264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).