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Tested Applications
| Positive FC detected in | rat splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-65228 targets CD45 in FC applications and shows reactivity with rat samples.
| Tested Reactivity | rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | N/A Predict reactive species |
| Full Name | protein tyrosine phosphatase, receptor type, C |
| Calculated Molecular Weight | 143 kDa |
| GenBank Accession Number | XM_006249910 |
| Gene Symbol | CD45 |
| Gene ID (NCBI) | 24699 |
| RRID | AB_3673994 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P04157 |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CD45 antibody PE-65228 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
