PE Anti-Rat CD45 (OX1)

CD45 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Rat

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

OX1

Cat no : PE-65228

Synonyms

CD45, L CA, Lca, Ptprc, RT7, T200

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Validation Data Gallery

• 

  FC experiment of rat splenocytes using PE-65228

  1X10^6 rat splenocytes were surface stained with 0.2 ug PE Anti-Rat CD45 (PE-65228, Clone:OX1) (red), or 0.2 ug PE Mouse IgG1 Isotype Control (MOPC-21) (PE-65124, Clone: MOPC-21) (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65228 targets CD45 in FC applications and shows reactivity with Rat samples.

• Tested Reactivity: Rat
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 143 kDa
• GenBank Accession Number: XM_006249910
• Gene Symbol: Ptprc
• Gene ID (NCBI): 24699
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).