FC experiment of wistar rat splenocytes using 65228-1-Ig
1X10^6 wistar rat splenocytes were surface stained with 0.5 ug Anti-Rat CD45 (65228-1-Ig, Clone:OX1) and FITC anti-Mouse IgG1 Antibody at dilution 1:100 (red), or 0.5 ug Control Antibody. Cells were not fixed.
1X10^6 wistar rat splenocytes were surface stained with 0.5 ug Anti-Rat CD45 (65228-1-Ig, Clone:OX1) and FITC anti-Mouse IgG1 Antibody at dilution 1:100 (red), or 0.5 ug Control Antibody. Cells were not fixed.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
65228-1-Ig targets CD45 in FC applications and shows reactivity with rat samples.
Store at 2-8°C. Stable for one year after shipment.
Background Information
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
FC experiment of wistar rat splenocytes using 65228-1-Ig
1X10^6 wistar rat splenocytes were surface stained with 0.5 ug Anti-Rat CD45 (65228-1-Ig, Clone:OX1) and FITC anti-Mouse IgG1 Antibody at dilution 1:100 (red), or 0.5 ug Control Antibody. Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD45 Monoclonal antibody
Catalog Number
65228-1-Ig
Citations
-
Dilutions
Applications
FC
Reactivity
rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
