PE Anti-Mouse CD45 (30-F11)

CD45 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

FC

Conjugate

PE Fluorescent Dye

Publications

2

CloneNo.

30-F11

Cat no : PE-65087

Synonyms

Ptprc, Leukocyte common antigen, LCA, B220, 30-F11

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Validation Data Gallery

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  FC experiment of C57BL/6 mouse splenocytes using PE-65087

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug PE Anti-Mouse CD45 (PE-65087, Clone:30-F11) (red), or 0.5 ug PE Rat IgG2b Isotype Control (LTF-2) (PE-65211, Clone: LTF-2) (blue). Cells were not fixed .

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

PE-65087 targets CD45 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse thymus or spleen
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC028512
• Gene Symbol: CD45
• Gene ID (NCBI): 19264
• RRID: AB_2883881
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Publications

Species	Application	Title
mouse	FC	Adv Sci (Weinh); Cancer-Associated Fibroblast-Induced Remodeling of Tumor Microenvironment in Recurrent Bladder Cancer; Authors - Ting Liang
mouse	FC	J Nanobiotechnology; The combination of modified acupuncture needle and melittin hydrogel as a novel therapeutic approach for rheumatoid arthritis treatment; Authors - Lisha Liu