Anti-Mouse CD45 (30-F11)

CD45 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

30-F11

Cat no : 65087-1-Ig

Synonyms

Ptprc, Leukocyte common antigen, LCA, B220, 30-F11

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using 65087-1-Ig

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45 (30-F11) (65087-1-Ig, Clone: 30-F11) or 0.25 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2), and FITC anti-rat IgG2b Antibody. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 65087-1-Ig

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45 (30-F11) (65087-1-Ig, Clone:30-F11) or 0.25 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2), and FITC anti-rat IgG2b Antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65087-1-Ig targets CD45 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse thymus or spleen
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC028512
• Gene Symbol: CD45
• Gene ID (NCBI): 19264
• RRID: AB_2918390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).