Anti-Human CD45 (2D1)

CD45 Monoclonal Antibody for IF, FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

IF, FC

Conjugate

Unconjugated

CloneNo.

2D1

Cat no : 65082-1-Ig

Synonyms

CD45, CD45R, EC:3.1.3.48, GP180, L CA, LCA, L-CA, Leukocyte common antigen, LY 5, LY5, PTPRC, Receptor-type tyrosine-protein phosphatase C, RPTPC, T200, 2D1, B220

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Validation Data Gallery

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  IF Staining of mouse spleen using 65082-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using CD45 antibody (65082-1-Ig, Clone: 2D1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of human peripheral blood lymphocytes using 65082-1-Ig

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD45 (65082-1-Ig, Clone: 2D1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

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Tested Applications

• Positive IF detected in: mouse spleen tissue
• Positive FC detected in: human peripheral blood lymphocytes

Recommended dilution

• Immunofluorescence (IF): IF : 1:125-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65082-1-Ig targets CD45 in IF, FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human PBMC
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC014239
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• RRID: AB_2918385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).