CoraLite® Plus 647 Anti-Human CD44 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using CL647-98333-3

  1x10^6 human PBMCs were surface stained with FITC Anti-Human CD3, and 5 ul CoraLite® Plus 647 Plus Anti-Human CD44 Rabbit RecAb (CL647-98333-3, Clone: 242345G9) or CoraLite® Plus 647 Plus Rabbit IgG Isotype Control RecAb CL647-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL647-98333-3

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD44 Rabbit RecAb (CL647-98333-3, Clone: 242345G9) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD44 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98333-3

Clone No.242345G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

242345G9, CD44 antigen, CDW44, CSPG8, ECMR III

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98333-3 targets CD44 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD44 fusion protein Eg1057
• Full Name: CD44 molecule (Indian blood group)
• Calculated Molecular Weight: 82 kDa
• GenBank Accession Number: NM_000610.3
• Gene Symbol: CD44
• Gene ID (NCBI): 960
• ENSEMBL Gene ID: ENSG00000026508
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16070-1
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.