Tested Applications
| Positive IF/ICC detected in | mouse splenocytes |
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-65117 targets CD44 in IF/ICC, FC applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Cited Reactivity | human |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Dexamethasone-induced myeloid leukemia M1 cells Predict reactive species |
| Full Name | CD44 antigen |
| GenBank Accession Number | BC051388 |
| Gene Symbol | Cd44 |
| Gene ID (NCBI) | 12505 |
| RRID | AB_2883189 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P15379 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD44 antibody CL488-65117 | Download protocol |
| IF protocol for CL Plus 488 CD44 antibody CL488-65117 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Mouse CD44 (IM7), catalog number CL488-65117, belongs to a product family with 11 total citations. For this specific conjugate, one citation was identified in Journal of Translational Medicine (IF 9.7), published in 2025. The study focused on colorectal cancer stemness, investigating a novel USP4 inhibitor that promotes β-catenin and Twist1 degradation. The associated research fields include oncology, cancer stem cell biology, and translational medicine.
| Species | Application | Title |
|---|---|---|
J Transl Med A novel USP4 inhibitor that suppresses colorectal cancer stemness by promoting β-catenin and Twist1 degradation |
