CoraLite® Plus 488 Anti-Mouse CD44 (IM7)

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  IF Staining of mouse splenocytes using CL488-65117

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488 CD44 antibody (CL488-65117, Clone: IM7 ) at dilution of 1:100.

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  FC experiment of mouse splenocytes using CL488-65117

  1X10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD44 (CL488-65117, Clone:IM7) or 0.5 ug CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2), and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

CD44 Monoclonal Antibody for IF/ICC, FC

Cat No. CL488-65117

Clone No.IM7

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

IF/ICC, FC

Synonyms

AU023126, AW121933, AW146109, Cd44, CD44 antigen, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, HERMES, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, Ly 24, MC56, MDU2, MDU3, MIC4, MUTCH I, Pgp 1, PGP I, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

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Tested Applications

• Positive IF/ICC detected in: mouse splenocytes
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65117 targets CD44 in IF/ICC, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Dexamethasone-induced myeloid leukemia M1 cells
• Full Name: CD44 antigen
• GenBank Accession Number: BC051388
• Gene Symbol: Cd44
• Gene ID (NCBI): 12505
• RRID: AB_2883189
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P15379
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.