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CoraLite® Plus 647 Anti-Mouse CD3 (17A2)

CD3 Monoclonal Antibody for IF, FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

17A2

Cat no : CL647-65077

Synonyms

AI504783, CD3, Cd3e, CD3epsilon, T3e

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse splenocytes using CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077)

    IF Staining of mouse splenocytes using CL647-65077

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) at dilution of 1:500.

  • Flow cytometry (FC) experiment of C57BL/6 mouse splenocytes using CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077)

    FC experiment of C57BL/6 mouse splenocytes using CL647-65077

    1X10^6 C57BL/6 mouse splenocytes were surface co-stained with PE Anti-Mouse CD4 (GK1.5) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (CL647-65077, Clone:17A2) or 0.5 ug CoraLite® Plus 647 Rat IgG2b Isotype Control (LTF-2) (CL647-65211, Clone: LTF-2). Cells were not fixed.

Tested Applications

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:250-1:1000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65077 targets CD3 in IF, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen γδTCR-positive T-T hybridoma D1
Full Name CD3 antigen, epsilon polypeptide
GenBank Accession NumberBC098236
Gene Symbol CD3ε
Gene ID (NCBI) 12501
RRIDAB_2883666
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 CD3 antibody CL647-65077Download protocol
FC protocol for CL Plus 647 CD3 antibody CL647-65077Download protocol
Standard Protocols
Click here to view our Standard Protocols