Anti-Mouse CD44 (IM7)

CD44 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Human, Mouse

Applications

IF/ICC, FC

Conjugate

Unconjugated

CloneNo.

IM7

Cat no : 65117-1-Ig

Synonyms

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

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Validation Data Gallery

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  IF Staining of HeLa using 65117-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CD44 antibody (65117-1-Ig, Clone: IM7 ) at dilution of 1:400 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

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  FC experiment of C57BL/c mouse splenocytes using 65117-1-Ig

  1X10^6 C57BL/c mouse splenocytes were surface co-stained with APC Anti-Mouse CD3 (17A2), and 0.5 ug Anti-Mouse CD44 (65117-1-Ig, Clone: IM7) or 0.5 ug isotype control, and FITC anti-rat IgG2b Antibody at dilution 1:100. Cells were not fixed.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:400

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65117-1-Ig targets CD44 in IF/ICC, FC applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Dexamethasone-induced myeloid leukemia M1 cells
• Full Name: CD44 antigen
• GenBank Accession Number: BC051388
• Gene Symbol: Cd44
• Gene ID (NCBI): 12505
• RRID: AB_2918413
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.