CoraLite® Plus 488-conjugated CD43 Monoclonal antibody

CD43 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A11D6

Cat no : CL488-66224

Synonyms

CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL488-66224

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66224 (CD43 antibody) at dilution of 1:50.

• 

  IF Staining of human tonsillitis using CL488-66224

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66224 (CD43 antibody) at dilution of 1:50.

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66224 targets CD43 in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD43 fusion protein Ag23595
• Full Name: sialophorin
• Calculated Molecular Weight: 400 aa, 43 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC012350
• Gene Symbol: CD43
• Gene ID (NCBI): 6693
• RRID: AB_2883288
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.