CD43 Monoclonal antibody

CD43 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2A11D6

Cat no : 66224-1-Ig

Synonyms

Leukocyte sialoglycoprotein, GPL115, GALGP, Galactoglycoprotein, CD43 cytoplasmic tail



Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inJurkat cells, human tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66224-1-Ig targets CD43 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD43 fusion protein Ag23595
Full Name sialophorin
Calculated Molecular Weight 400 aa, 43 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC012350
Gene Symbol CD43
Gene ID (NCBI) 6693
RRIDAB_2881615
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.

Protocols

Product Specific Protocols
WB protocol for CD43 antibody 66224-1-IgDownload protocol
IHC protocol for CD43 antibody 66224-1-IgDownload protocol
IF protocol for CD43 antibody 66224-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols