Anti-Mouse CD40 Rabbit Recombinant Antibody, PBS Only

CD40 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240874G8

Cat no : 98122-1-PBS

Synonyms

Tnfrsf5, CD40 antigen, Bp50, 240874G8

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Validation Data Gallery

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  FC experiment of mouse splenocytes using 98122-1-RR (same clone as 98122-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR, Clone: 240874G8) (red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98122-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98122-1-RR (same clone as 98122-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR, Clone: 240874G8) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD19 (1D3) (APC-65290, Clone: 1D3). Cells were not fixed. This data was developed using the same antibody clone with 98122-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98122-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98122-1-RR against Mouse CD40 were performed. The affinity constant is 0.997 nM.

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Product Information

98122-1-PBS targets CD40 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD40 antigen
• Calculated Molecular Weight: 32 kDa
• GenBank Accession Number: NM_011611.2
• Gene Symbol: Cd40
• Gene ID (NCBI): 21939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).