Anti-Mouse CD40 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using 98122-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR, Clone: 240874G8) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD19 (1D3) (APC-65290, Clone: 1D3). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98122-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR, Clone: 240874G8) (red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98122-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98122-1-RR against Mouse CD40 were performed. The affinity constant is 0.997 nM.

CD40 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98122-1-RR

Clone No.240874G8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Tnfrsf5, CD40 antigen, Bp50, 240874G8

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98122-1-RR targets CD40 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD40 antigen
• Calculated Molecular Weight: 32 kDa
• GenBank Accession Number: NM_011611.2
• Gene Symbol: Cd40
• Gene ID (NCBI): 21939
• RRID: AB_3672269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P27512-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).