Anti-Mouse CD4 (RM4-5)

CD4 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

RM4-5

Cat no : 65141-1-Ig

Synonyms

Cd4, CD4 antigen, L3T4, Ly 4

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using 65141-1-Ig

  1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3, and 0.2 ug Anti-Mouse CD4 (65141-1-Ig, Clone:RM4-5) and FITC-Goat anti-Mouse IgG at dilution 1:100 or 0.2 ug Isotype Control and FITC-Goat anti-Mouse IgG at dilution 1:100. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65141-1-Ig targets CD4 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: BALB/c mouse thymocytes
• Full Name: CD4 antigen
• GenBank Accession Number: BC039137
• Gene Symbol: Cd4
• Gene ID (NCBI): 12504
• RRID: AB_2918427
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.1% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).