APC-Cyanine7 Anti-Mouse CD4 (GK1.5)

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  FC experiment of mouse splenocytes using AY7-65104

  1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3, and 0.2 ug APC-Cyanine7 Anti-Mouse CD4 (GK1.5) (AY7-65104, Clone: GK1.5) or APC-Cyanine7 Rat IgG2b Isotype Control (LTF-2) (AY7-65211, Clone: LTF-2). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using AY7-65104

  1x10^6 mouse splenocytes were surface stained with 0.2 ug APC-Cyanine7 Anti-Mouse CD4 (GK1.5) (AY7-65104, Clone: GK1.5) (red) or APC-Cyanine7 Rat IgG2b Isotype Control (LTF-2) (AY7-65211, Clone: LTF-2) (blue). Cells were not fixed.

CD4 Monoclonal Antibody for FC

Cat No. AY7-65104

Clone No.GK1.5

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

FC

Synonyms

Ly 4, L3T4, GK1.5, CD4 antigen

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

AY7-65104 targets CD4 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse CTL clone V4
• Full Name: CD4 antigen
• GenBank Accession Number: BC039137
• Gene Symbol: Cd4
• Gene ID (NCBI): 12504
• RRID: AB_3672360
• Conjugate: APC-Cyanine7 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 778 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P06332
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).