CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5)

CD4 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

IF/ICC, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Publications

2

CloneNo.

GK1.5

Cat no : CL647-65104

Synonyms

Ly 4, L3T4, GK1.5, CD4 antigen

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Validation Data Gallery

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  IF Staining of mouse splenocytes using CL647-65104

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) at dilution of 1:500.

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  FC experiment of mouse splenocytes using CL647-65104

  1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) or CoraLite® Plus 647-Conjugated rat IgG2b isotype control. Cells were not fixed.

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Tested Applications

• Positive IF/ICC detected in: mouse splenocytes
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

CL647-65104 targets CD4 in IF/ICC, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse CTL clone V4
• Full Name: CD4 antigen
• GenBank Accession Number: BC039137
• Gene Symbol: Cd4
• Gene ID (NCBI): 12504
• RRID: AB_2883682
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publications

Species	Application	Title
	IF	Front Chem; Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency; Authors - Yinghui Shang
mouse	IF	J Transl Med; HMGB1/TREM2 positive feedback loop drives the development of radioresistance and immune escape of glioblastoma by regulating TLR4/Akt signaling; Authors - Hui Qiu