CoraLite® Plus 405 Anti-Human CD39/ENTPD1 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using CL405-98102

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD4, and 5 ul CoraLite® Plus 405 Anti-Human CD39/ENTPD1 Rabbit RecAb (CL405-98102, Clone:241292G7) or CoraLite® Plus 405 Rabbit IgG Isotype Control RecAb (CL405-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL405-98102

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD39/ENTPD1 Rabbit RecAb (CL405-98102, Clone:241292G7)(red) or CoraLite® Plus 405 Rabbit IgG Isotype Control RecAb (CL405-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD39/ENTPD1 {ptg:Brand} Recombinant Antibody for FC

Cat No. CL405-98102

Clone No.241292G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

ENTPD1, EC:3.6.1.5, CD39, ATP-DPH, ATPDase

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is N/A per 10^6 cells in a 100 µl suspension or N/A per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL405-98102 targets CD39/ENTPD1 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: ectonucleoside triphosphate diphosphohydrolase 1
• Calculated Molecular Weight: 58 kDa
• GenBank Accession Number: BC047664
• Gene Symbol: CD39
• Gene ID (NCBI): 953
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49961
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

ENTPD1 (Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers (PMID: 9733785 ).