Anti-Mouse CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody, PBS Only

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  FC experiment of mouse splenocytes using 98113-1-RR (same clone as 98113-1-PBS)

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98113-1-RR, Clone: 241012G10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD4. Cells were not fixed. This data was developed using the same antibody clone with 98113-1-PBS in a different storage buffer formulation.

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  FC experiment of mouse splenocytes using 98113-1-RR (same clone as 98113-1-PBS)

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98113-1-RR, Clone:241012G10) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98113-1-RR, Clone: 241012G10) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98113-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98113-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98113-1-RR against Mouse CD30 Ligand/TNFSF8 were performed. The affinity constant is 0.342 nM.

CD30 Ligand/TNFSF8 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98113-1-PBS

Clone No.241012G10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Tnfsf8, Tumor necrosis factor ligand superfamily member 8, CD30-L, CD30 ligand, 241012G10

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Product Information

98113-1-PBS targets CD30 Ligand/TNFSF8 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: tumor necrosis factor (ligand) superfamily, member 8
• Calculated Molecular Weight: 27 kDa
• GenBank Accession Number: NM_009403.3
• Gene Symbol: Tnfsf8
• Gene ID (NCBI): 21949
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P32972
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD30 Ligand (CD30L), also named as TNFSF8 or CD153, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) superfamily (PMID: 8391931). It is expressed on the surface of activated T cells, B cells, monocytes, macrophages, eosinophils, neutrophils, and mast cells (PMID: 26090498). CD30L interacts with its receptor, CD30, which is a cell surface protein expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin lymphoma and anaplastic large cell lymphomas (PMID: 28885612). The CD30/CD30L signaling is involved in pleiotropic downstream effects including differentiation, cell survival and death, NFkB activation, and production of cytokines (PMID: 38534788).