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Anti-Mouse CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody

CD30 Ligand/TNFSF8 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241012G10

Cat no : 98113-1-RR

Synonyms

Tnfsf8, Tumor necrosis factor ligand superfamily member 8, CD30-L, CD30 ligand, 241012G10

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CD30 Ligand/TNFSF8 Rabbit Recombinant (98113-1-RR)

    FC experiment of mouse splenocytes using 98113-1-RR

    1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98113-1-RR, Clone: 241012G10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD4. Cells were not fixed.

  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CD30 Ligand/TNFSF8 Rabbit Recombinant (98113-1-RR)

    FC experiment of mouse splenocytes using 98113-1-RR

    1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98113-1-RR, Clone:241012G10) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98113-1-RR, Clone: 241012G10) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98113-1-RR

    Affinity and Kinetic Characterization of 98113-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98113-1-RR against Mouse CD30 Ligand/TNFSF8 were performed. The affinity constant is 0.342 nM.

Tested Applications

Positive FC detected inAnti-CD3/CD28 treated mouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98113-1-RR is CD30 Ligand/TNFSF8 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name tumor necrosis factor (ligand) superfamily, member 8
Calculated Molecular Weight27 kDa
GenBank Accession NumberNM_009403.3
Gene Symbol Tnfsf8
Gene ID (NCBI) 21949
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD30 Ligand, also named as TNFSF8 and CD153, is a member of the tumor necrosis factor (TNF) receptor superfamily, and is a surface antigen used as a clinical marker for Hodgkin lymphoma and related hematologic malignancies. It induces the proliferation of T-cells. CD30L enhanced the proliferation of CD3-activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.

Protocols

Product Specific Protocols
FC protocol for CD30 Ligand/TNFSF8 antibody 98113-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols