Anti-Mouse CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody

CD30 Ligand/TNFSF8 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241012G10

Cat no : 98113-1-RR

Synonyms

Tnfsf8, Tumor necrosis factor ligand superfamily member 8, CD30-L, CD30 ligand, 241012G10

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Validation Data Gallery

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  FC experiment of mouse splenocytes using 98113-1-RR

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98113-1-RR, Clone: 241012G10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD4. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98113-1-RR

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98113-1-RR, Clone:241012G10) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98113-1-RR, Clone: 241012G10) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98113-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98113-1-RR against Mouse CD30 Ligand/TNFSF8 were performed. The affinity constant is 0.342 nM.

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98113-1-RR is CD30 Ligand/TNFSF8 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: tumor necrosis factor (ligand) superfamily, member 8
• Calculated Molecular Weight: 27 kDa
• GenBank Accession Number: NM_009403.3
• Gene Symbol: Tnfsf8
• Gene ID (NCBI): 21949
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD30 Ligand, also named as TNFSF8 and CD153, is a member of the tumor necrosis factor (TNF) receptor superfamily, and is a surface antigen used as a clinical marker for Hodgkin lymphoma and related hematologic malignancies. It induces the proliferation of T-cells. CD30L enhanced the proliferation of CD3-activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.