Biotin Anti-Human CD27 (O323) Mouse IgG2a Recombinant Antibody

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  FC experiment of human PBMCs using Biotin-65627

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD27 (O323) Mouse IgG2a RecAb (Biotin-65627, Clone: O323) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with APC Anti-Human CD3. Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-65627

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD27 (O323) Mouse IgG2a RecAb (Biotin-65627, Clone: O323) (red) and CoraLite®488-conjugated streptavidin, or unstained (blue). Cells were not fixed.

CD27 Uni-rAb^TM Recombinant Antibody for FC

Cat No. Biotin-65627

Clone No.O323

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

T cell activation antigen CD27, S152, O323, CD27L receptor, CD27 molecule

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65627 targets CD27 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD27 molecule
• Calculated Molecular Weight: 29 kDa
• GenBank Accession Number: BC012160
• Gene Symbol: CD27
• Gene ID (NCBI): 939
• ENSEMBL Gene ID: ENSG00000139193
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.