Anti-Human CD27 (O323) Mouse IgG2a Recombinant Antibody

CD27 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

O323

Cat no : 65627-1-MR

Synonyms

T cell activation antigen CD27, S152, O323, CD27L receptor, CD27 molecule

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Validation Data Gallery

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  FC experiment of human PBMCs using 65627-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD27 (O323) Mouse IgG2a Recombinant Antibody (65627-1-MR, Clone:O323) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65627-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD27 (O323) Mouse IgG2a Recombinant Antibody (65627-1-MR, Clone: O323) (red) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65627-1-MR targets CD27 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD27 molecule
• Calculated Molecular Weight: 29 kDa
• GenBank Accession Number: BC012160
• Gene Symbol: CD27
• Gene ID (NCBI): 939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.