APC Anti-Mouse CD25 (PC61.5)

CD25 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, lambda

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

APC Fluorescent Dye

Publications

3

CloneNo.

PC61.5

Cat no : APC-65137

Synonyms

CD25, Il2r, Il2ra, Ly 43

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Validation Data Gallery

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  FC experiment of BALB/C mouse splenocytes using APC-65137

  1X10^6 untreated or anti-CD3/CD28 treated (2 days) mouse splenocytes were co-surface stained with FITC Plus Anti-Mouse CD4 (GK1.5) and 0.25 ug APC Anti-Mouse CD25 (APC-65137, Clone:PC61.5) or 0.25 ug APC Rat IgG1 Isotype Control (HRPN). Cells were not fixed.

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated 2days mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 3 publications below

Product Information

APC-65137 targets CD25 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG1, lambda
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: interleukin 2 receptor, alpha chain
• GenBank Accession Number: BC114437
• Gene Symbol: CD25
• Gene ID (NCBI): 16184
• RRID: AB_2883018
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).

Publications

Species	Application	Title
mouse	FC	Phytomedicine; Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.; Authors - Li Wu
mouse	FC	Acta Biomater; Injectable microenvironment-responsive hydrogels with redox-activatable supramolecular prodrugs mediate ferroptosis-immunotherapy for postoperative tumor treatment; Authors - Zhuo Cheng
mouse	FC	J Neuroinflammation; 27-hydroxycholesterol causes cognitive deficits by disturbing Th17/Treg balance and the related immune responses in mild cognitive impairment patients and C57BL/6J mice; Authors - Tao Wang