CD23 Recombinant antibody, PBS Only (Detector)

CD23 Uni-rAb^TM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241269B10

Cat no : 84024-2-PBS

Synonyms

C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2

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Validation Data Gallery

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  WB analysis using 84024-2-RR (same clone as 84024-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84024-2-RR (CD23,FCER2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84024-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84024-2-RR (same clone as 84024-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84024-2-RR (CD23 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84024-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84024-2-RR (same clone as 84024-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84024-2-RR (CD23 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84024-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00958-1

  Cytometric bead array standard curve of MP00958-1, CD23 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84024-1-PBS. Detection antibody: 84024-2-PBS. Standard: RP02343. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 84024-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83996-4-RR against Human CD23 were performed. The affinity constant is 3.66 nM.

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Product Information

84024-2-PBS targets CD23 as part of a matched antibody pair:

MP00958-1: 84024-1-PBS capture and 84024-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Calculated Molecular Weight: 321 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC064417
• Gene Symbol: CD23
• Gene ID (NCBI): 2208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD23, also known as low-affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centers, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells