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CD23 Recombinant antibody

CD23 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241269B10

Cat no : 84024-2-RR

Synonyms

C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD23 Recombinant antibody (84024-2-RR)

    WB analysis using 84024-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84024-2-RR (CD23,FCER2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD23 Recombinant antibody (84024-2-RR)

    IHC staining of human tonsillitis using 84024-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84024-2-RR (CD23 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD23 Recombinant antibody (84024-2-RR)

    IHC staining of human tonsillitis using 84024-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84024-2-RR (CD23 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84024-2-RR

    Affinity and Kinetic Characterization of 84024-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83996-4-RR against Human CD23 were performed. The affinity constant is 3.66 nM.

Tested Applications

Positive WB detected inRaji cells, Ramos cells, Daudi cells, U-937 cells, NIH/3T3 cells, RAW 264.7 cells, mouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84024-2-RR targets CD23 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Calculated Molecular Weight 321 aa, 36 kDa
Observed Molecular Weight36 kDa
GenBank Accession NumberBC064417
Gene Symbol CD23
Gene ID (NCBI) 2208
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD23, also known as low-affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centers, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells

Protocols

Product Specific Protocols
WB protocol for CD23 antibody 84024-2-RRDownload protocol
IHC protocol for CD23 antibody 84024-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols