CD23 Recombinant antibody, PBS Only (Capture/Detector)

CD23 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241269B5

Cat no : 84024-1-PBS

Synonyms

C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00958-3

  Sandwich ELISA standard curve of MP00958-3, Human CD23 Recombinant Matched Antibody Pair - PBS only. 84024-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02343. 84024-1-PBS was HRP conjugated as the detection antibody. Range: 7.81-500 pg/mL

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  Sample test of MP00958-3

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean CD23 concentration was determined to be 16.3 pg/mL in unstimulated PBMC supernatant, 444.73 pg/mL in PHA stimulated PBMC supernatant. Raji human Burkitt’s lymphoma cells were cultured in RPMI supplemented with 10% fetal bovine serum, 2mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The mean CD23 concentration was determined to be 742.6 pg/mL Raji cell culture supernatants.

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  Sample test of MP00958-3

  Serum of sixteen individual healthy human donors was measured. The human CD23 concentration of detected samples was determined to be 651.0 pg/mL with a range of 259.5 - 1,165.0 pg/mL.

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  Cytometric bead array standard curve of MP00958-1

  Cytometric bead array standard curve of MP00958-1, CD23 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84024-1-PBS. Detection antibody: 84024-2-PBS. Standard: RP02343. Range: 0.156-20 ng/mL.

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  Cytometric bead array standard curve of MP00958-2

  Cytometric bead array standard curve of MP00958-2, CD23 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84024-1-PBS. Detection antibody: 84024-3-PBS. Standard: RP02343. Range: 0.156-20 ng/mL.

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Product Information

84024-1-PBS targets CD23 as part of a matched antibody pair:

MP00958-1: 84024-1-PBS capture and 84024-2-PBS detection (validated in Cytometric bead array)

MP00958-2: 84024-1-PBS capture and 84024-3-PBS detection (validated in Cytometric bead array)

MP00958-3: 84024-3-PBS capture and 84024-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Calculated Molecular Weight: 321 aa, 36 kDa
• GenBank Accession Number: BC064417
• Gene Symbol: CD23
• Gene ID (NCBI): 2208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.