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CD23 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 7.81-500 pg/mL

Cat No : MP00958-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00958-3, Human CD23 Recombinant Matched Antibody Pair - PBS only. 84024-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02343. 84024-1-PBS was HRP conjugated as the detection antibody. Range: 7.81-500 pg/mL

    Sandwich ELISA standard curve of MP00958-3

    Sandwich ELISA standard curve of MP00958-3, Human CD23 Recombinant Matched Antibody Pair - PBS only. 84024-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02343. 84024-1-PBS was HRP conjugated as the detection antibody. Range: 7.81-500 pg/mL

  • Serum of sixteen individual healthy human donors was measured. The human CD23 concentration of detected samples was determined to be 651.0 pg/mL with a range of 259.5 - 1,165.0 pg/mL.

    Sample test of MP00958-3

    Serum of sixteen individual healthy human donors was measured. The human CD23 concentration of detected samples was determined to be 651.0 pg/mL with a range of 259.5 - 1,165.0 pg/mL.

  • Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean CD23 concentration was determined to be 16.3 pg/mL in unstimulated PBMC supernatant, 444.73 pg/mL in PHA stimulated PBMC supernatant. Raji human Burkitt’s lymphoma cells were cultured in RPMI supplemented with 10% fetal bovine serum, 2mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The mean CD23 concentration was determined to be 742.6 pg/mL Raji cell culture supernatants.

    Sample test of MP00958-3

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean CD23 concentration was determined to be 16.3 pg/mL in unstimulated PBMC supernatant, 444.73 pg/mL in PHA stimulated PBMC supernatant. Raji human Burkitt’s lymphoma cells were cultured in RPMI supplemented with 10% fetal bovine serum, 2mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The mean CD23 concentration was determined to be 742.6 pg/mL Raji cell culture supernatants.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00958-3 targets CD23 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 7.81-500 pg/mL (Sandwich ELISA);

Capture antibody: CD23 Recombinant antibody, PBS Only (Capture/Detector) 84024-3-PBS (241269C8). 100 μg. Concentration 1 mgl/ml.

Detection antibody: CD23 Recombinant antibody, PBS Only (Capture/Detector) 84024-1-PBS (241269B5). 100 μg. Concentration 1 mgl/ml.

Alternative CD23 matched antibody pairs: MP00958-1, MP00958-2

Matched pair protein standard: RP02343

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84024-3-PBS
Tested Reactivity human
Immunogen Recombinant Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Synonyms C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2
GenBank accession numberBC064417
Gene symbol CD23
UniprotP06734
Gene ID (NCBI) 2208
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84024-1-PBS
Tested Reactivity human
Immunogen Recombinant Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Synonyms C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2
GenBank accession numberBC064417
Gene symbol CD23
UniprotP06734
Gene ID (NCBI) 2208
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.