CoraLite® Plus 647 Anti-Human CD209 Rabbit Recombinant Antibody

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  FC experiment of human monocyte-derived immature dendritic cells using CL647-98156

  1x10^6 human monocyte-derived immature dendritic cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD209 Rabbit RecAb (CL647-98156, Clone: 240907A4) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD209 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98156

Clone No.240907A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

240907A4, CDSIGN, CLEC4L, C-type lectin domain family 4 member L, DC SIGN

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Tested Applications

• Positive FC detected in: human monocyte-derived immature dendritic cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98156 targets CD209 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD209 fusion protein Eg0062
• Full Name: CD209 molecule
• Calculated Molecular Weight: 404 aa, 45.7 kDa
• GenBank Accession Number: BC110615
• Gene Symbol: CD209
• Gene ID (NCBI): 30835
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NNX6
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD209 (DC-SIGN, CLEC4L) is a C-type lectin receptor that is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens, including bacteria, viruses, and parasites. CD209 is preferentially expressed on dendritic cells (DCs). It mediates transient adhesion of DCs with T cells. It has been reported that CD209 plays a role in capture and transmission of HIV from DC to T cells (PMID: 10721995).