CoraLite® Plus 488 Anti-Mouse CD200 Rabbit Recombinant Antibody

CD200 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240391A6

Cat no : CL488-98039

Synonyms

OX2, Mox2, CD200 antigen, 240391A6

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Validation Data Gallery

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  FC experiment of mouse splenocytes using CL488-98039

  1x10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD45R and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD200 Rabbit Recombinant Antibody (CL488-98039, Clone:240391A6), or Isotype Control. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL488-98039

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD200 Rabbit Recombinant Antibody (CL488-98039, Clone:240391A6) (red), or Isotype Control (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-98039 is CD200 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD200 antigen
• Calculated Molecular Weight: 31 kDa
• Gene Symbol: Cd200
• Gene ID (NCBI): 17470
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).