Anti-Mouse CD200 Rabbit Recombinant Antibody

CD200 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240391A6

Cat no : 98039-1-RR

Synonyms

OX2, Mox2, CD200 antigen, 240391A6

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using 98039-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD200 Rabbit Recombinant Antibody (98039-1-RR, Clone: 240391A6) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD45R (B220) (RA3-6B2) (CL647-65139, Clone: RA3-6B2). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98039-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD200 Rabbit Recombinant Antibody (98039-1-RR, Clone: 240391A6) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98039-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98039-1-RR against Mouse CD200 were performed. The affinity constant is 0.879 nM.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98039-1-RR is CD200 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD200 antigen
• Calculated Molecular Weight: 31 kDa
• Gene Symbol: Cd200
• Gene ID (NCBI): 17470
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).