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CoraLite® Plus 647 Anti-Mouse CD45R (B220) (RA3-6B2)

CD45R (B220) Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF/ICC, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

RA3-6B2

Cat no : CL647-65139

Synonyms

CD45R, Cd45, Ptprc, Leukocyte common antigen, LCA

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse splenocytes using CoraLite® Plus 647 Anti-Mouse CD45R (B220) (RA3-6B (CL647-65139)

    IF Staining of mouse splenocytes using CL647-65139

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated CD45R (B220) antibody (CL647-65139, Clone: RA3-6B2, red) at dilution of 1:400, and CoraLite®488-conjugated CD3 antibody (CL488-65077, Clone: 17A2, green).

  • Flow cytometry (FC) experiment of C57BL/c mouse splenocytes using CoraLite® Plus 647 Anti-Mouse CD45R (B220) (RA3-6B (CL647-65139)

    FC experiment of C57BL/c mouse splenocytes using CL647-65139

    1x10^6 C57BL/c mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD45R (B220) (CL647-65139, Clone:RA3-6B2) or 0.25 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3). Cells were co-stained with PE Anti-Mouse CD3 (17A2) (PE-65077, Clone: 17A2). Cells were not fixed.

Tested Applications

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65139 targets CD45R (B220) in IF/ICC, FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Abelson murine leukemia virus-induced pre-B tumor cells
Full Name protein tyrosine phosphatase, receptor type, C
GenBank Accession NumberBC028512
Gene Symbol CD45
Gene ID (NCBI) 19264
RRIDAB_2883705
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 CD45R (B220) antibody CL647-65139Download protocol
FC protocol for CL Plus 647 CD45R (B220) antibody CL647-65139Download protocol
Standard Protocols
Click here to view our Standard Protocols